[Comparison of moderate and high volume aerobic training on gene expression of uncoupling protein 1 [UCP-1] in subcutaneous white adipose tissue of wistar rats]

Introduction: The aim of this study was to investigate the effect of moderate and high volume aerobic training on the expression of Uncoupling Protein 1[UCP-1] gene in subcutaneous WAT [sub-WAT]

Materials and Methods: Twenty-four rats were assigned randomly into three groups: 1] control [n=8] 2] moderate-volume aerobic training [n=8] and 3] high-volume aerobic training [n=8]. Subjects of training groups underwent continuous aerobic training on the treadmill for 8 weeks, 5 sessions per week at two different volumes of training. The Real Time-PCR method was used to measure the expression ratio of UCP-1 gene

Results: Data showed that although the expression ratio of UCP1 gene in the moderate volume aerobic training group was significantly higher than control group [P=0.014], its expression ratio in the high volume aerobic training group did not differ significantly from controls [P=0.36]; neither was the gene expression ratio different between moderate and high volume aerobic training groups [P=0.59]

Conclusion: Results indicate that moderate volume aerobic training, had an obvious effect in inducing UCP1 gene expression in subcutaneous adipose tissue, while the high volume aerobic training did not. Thus, increasing the volume of aerobic training to high levels may not be a key factor in promoting the non-shivering theremogenesis of sub-WAT

[ effect of resistance training on plasma and skeletal muscles sphingosine-1-phosphate levels of male Wistar rat]

Sphingosine 1-phosphate [S1P] is a bioactive platelet-derived sphingolipid that is involved in regulation of proliferation, differentiation, hypertrophy and anti-apoptosis of cells and activation of satellite cells. The purpose of present study was to examine the effect of resistance training on S1P levels of plasma and skeletal muscles in male Wistar rats. Twenty four 8-week-old male Wistar rats were used in this study. The initial body weight of rats was 190 to 250 gr. All animals were maintained in pairs in an environmentally controlled room at 22°C, 12:12-h photoperiod cycle and allowed normal cage activity. The animals were fed standard rat chow and water ad libitum. After a week of acclimation to the animal facility, the rats were assigned randomly to a control [N=12] or training [N=12] group. Resistance training was done using a 1 meter height ladder with 2 cm grid with an 85 degree incline, and weights attached to rat's tails. The content of sphingosine-1- phosphate [S1P] present in the chloroform layer was determined by means of high performance liquid chromatography [HPLC]. Resistance exercise training increased the total content of S1P in FHL [fast-twitch] [P=0.003] and soleus [slow-twitch] [P=0.008] muscles and plasma [P=0.001] in comparison with control group. It is concluded that resistance exercise training strongly affects the S1P content in fast and slow twitch muscles and plasma